“What mixer will fit my app? I want to optimize it to get as much mixing as I can without creating too much noise or dead volume. How do I know what will be the optimal mixer volume?”
There is no one correct answer for this question. Our Static Mixer Selection Chart will provide a good estimate. However, to refine the solution the user will need to keep in mind that more mixing volume will create more delay time and mixer noise. Not enough mixing may result in an unsatisfactory result. To optimize the mixer volume, the scientist must tune the mixer volume to meet performance specifications of the system method. To save time and development effort, you might consider the new ASI Static Mixer Development Kit.
“How do I select the best fit mixer volume for my application?
HPLC – select smallest volume to limit dwell time & peak dispersion
Non-reactive reagent mixing – select by largest flow rate
Reactive mixing – select larger volume – up to 10x flow of rate-limiting reagent.
Other – contact ASI!
DWELL volume (supply app note for measuring dwell volume)
If dwell volume of system is known, then pick a mixer with that volume.
If dwell volume is not known, then determine it (see app note) and match the experimentally determined volume.
There is no one correct answer for this question. Our Static Mixer Selection Chart will provide a good estimate. However, to refine the solution the user will need to keep in mind that more mixing volume will create more delay time and mixer noise. Not enough mixing may result in an unsatisfactory result.
I would like to incorporate 50 µL mixer in a HPLC system to mix two solutions with a 6:1 concentration ratio (0.2 M permanganate at pH 14 + 0.03 M sodium iodide, both aqueous solutions), working between 0.5 mL/min. and 2 mL/min. flow rate.
Could you please inform me about the approximate degree of mixing achieved in this case?
PEEK and chemical compatibility are easy — we have PEEK mixers and they are compatible with your reagents.
More mixing volume will create more delay time and more noise. Not enough mixing may result in a failed experiment. We can’t accurately predict if 50 µL is enough for your application. Based on flow only, you might consider a 350 µL mixer. However, you must confirm if this general approach will meet your performance specifications.
I was wondering if you could tell me if ASI Dynamic Mixers are compatible with Agilent 1100/1200/1260 HPLC.
Our Dynamic mixers were developed for use with the Agilent 1100 series pumps, especially when using TFA or other difficult mixing applications.
- When mixing noise is limiting, use Flow Rate, Column 1
- Mixing delay volume is sometimes referred to as dead volume. It affects peak shape or dispersion. Use Flow Rate, Column 2 when this is critical.