Mass directed fraction collection:
The addition of a Makeup-Flow Splitter can substantially reduce peak band broadening, while allowing a small portion of the outlet flow from a preparative HPLC column to be split off and then combined and diluted with a makeup-flow. The resulting makeup-flow is used by a detector, typically a Mass Spectrometer, UV, or other detector to trigger fraction collection from the remaining preparative flow. The split ratio of ASI Makeup-Flow Splitter is not influenced by the solvent composition – so reliable fraction collection is assured. The ASI Makeup-Flow Splitter will continue working for years with minimal maintenance. For these reasons the ASI Adjustable/Fixed Makeup-Flow Splitter is frequently used by major HPLC systems manufacturers for their splitter applications.