HyperShear™ Static Mixers

  • Static Mixers are available in Standard HPLC, Ultra HPLC, Biocompatible PEEK and In-Line Combo Series
  • Standard HPLC Mixers are constructed from 316 stainless steel with volumes ranging from 0.5 μL to 15.0 mL and with pressure to 6,000 PSI.
  • Ultra HPLC Mixers are constructed from 316 stainless steel with volumes ranging from 0.5 µL to 1.5 mL and pressure to 18,000 PSI.
  • Biocompatible Mixers are constructed from PEEK as wetted material with volumes ranging from 0.5 μL to 1.5 mL and with pressure to 3,000 PSI.
  • In-Line Combo Mixers are suitable for seeking to optimize mixing volumes at flow rates below 100 μL/min. and have the ability to customize a mixing volume which is perfectly tuned to match the separation system.
  • ASI Hypershear Mixer enables pharmaceutical assay.  read this article
  • Static Mixer Development Kits for the equipment manufacturer or the scientist that needs to optimize a separation or process, are available.


Static Mixer Application Notes and Volume Selection Guide in PDF and Static Mixer Selection Chart below on this page

ASI vs Competitor Comparison

Static Mixer Performance Data 1 - 8

Static Mixer Performance Data 9 - 10  Shimadzu LC-10ADVP pump data

Static Mixer Performance Data 11  ASI Hypershear Mixer enables pharmaceutical assay.


Chromatography Forum, HPLC Static Mixers  Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

Journal of Chromatography, ASI 250 μL Static Mixer  The application of ASI 250 µL mixer to neutralize an antibody – protein system 


Linear Gradients - High Pressure Mixing

The best choice is usually a compromise between delay volume and mixing noise - more mixing volume will reduce mixing noise but at the cost of more delay volume.  The upper limits to mixing volume will be the maximum delay time that can be tolerated, and possible distortion (tailing) of the gradient at the beginning and end of the gradient. The lower limit will be defined by the amount of mixing noise that can be tolerated. The following cartridge volumes are a compromise between these two limits.


For illustration ONLY

1. When mixing noise is limiting, use Flow Rate, Column 1

2. Mixing delay volume is sometimes referred to as dead volume. It affects peak shape or dispersion. Use Flow Rate, Column 2 when this is critical.

Flow RateMaximum Operating Pressure
Column 1 Column 2   Product Links
The lower limit will be defined by the amount of mixing noise that can be tolerated The upper limit will be the maximum delay time that can be tolerated ASI Mixer Cartridge Volume SST 6K PSI SST 18K PSI Biocompatible 3K PSI
  < 1 µL/min. 0.5 µL 40X-000.5 40X-000.5HP 40X-000.5B
0.5 µL/min. 2 µL/min. 1 µL 40X-0001 40X-0001HP 40X-0001B
1 µL/min. 5 µL/min. 2 µL 40X-0002 40X-0002HP 40X-0002B
2 µL/min. 10 µL/min. 5 µL 40X-0005 40X-0005HP 40X-0005B
5 µL/min. 20 µL/min. 10 µL 40X-0010 40X-0010HP 40X-0010B
10 µL/min. 50 µL/min. 25 µL 40X-0025 40X-0025HP 40X-0025B
20 µL/min. 100 µL/min. 50 µL 41X-0050 41X-0050HP 41X-0050B
40 µL/min. 160 µL/min. 80 µL 41X-0080 41X-0080HP  
60 µL/min. 200 µL/min. 100 µL 41X-0100 41X-0100HP 41X-0100B
100 µL/min. 250 µL/min. 150 µL 41X-0150 41X-0150HP 41X-0150B
150 µL/min. 400 µL/min. 200 µL 41X-0200 41X-0200HP 41X-0200B
200 µL/min. 500 µL/min. 250 µL 41X-0250 41X-0250HP 41X-0250B
500 µL/min. 2 mL/min. 350 µL 42X-0350 42X-0350HP 42X-0350B
1 mL/min. 5 mL/min. 500 µL 42X-0500 42X-0500HP 42X-0500B
2 mL/min. 10 mL/min. 800 µL 43X-0800 43X-0800HP 43X-0800B
5 mL/min. 20 mL/min. 1.0 mL 43X-1000 43X-1000HP 43X-1000B
10 mL/min. 30 mL/min. 1.5 mL 43X-1500 43X-1500HP 43X-1500B
20 mL/min. 50 mL/min. 3.0 mL 45X-30X0    
30 mL/min. 80 mL/min. 6.0 mL 45X-60X0    
50 mL/min. 100 mL/min. 9.0 mL 45X-90X0    
80 mL/min. 1 L/min. 15 mL 45X-150X0