QuickSplit™ Adjustable Makeup-Flow Splitter
Adjustable Makeup-Flow Splitter Performance
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The chromatographic data below compares the dispersion (band broadening), measured as variance, at 10 μL/min. between an ideal system with direct flow (Figure 19) and a split system incorporating the ASI QuickSplit™ Adjustable Makeup-Flow Splitter (Figure 20). Split flow variance is measured with and without the addition of a small amount of makeup flow. The data proves conclusively that ASI splitter dead volume does not contribute significantly to overall system dispersion. It also demonstrates the advantages of adding a makeup flow not only to improve peak shape but acts as an additional tool to optimize the timing sequence between Mass Spectrometer detector and the fraction collector.
| Conditions: | Variance Calculation: |
| HPLC System: Shimadzu LC10 AD VP Detector: UV @254 on column Solvent: Water Injection vol: direct 600nL, split 135µL |
Variance = Sigma2 = (Wh x F)2 = µL2 W = Peak width at half height F = Flow rate |
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| Figure 19 | Figure 20 |
