
PrimeLine™ Micro Pump Performance Data
Figure 1
HPLC System Configuration:
ASI PrimeLine Model 500G Micro Head
Detection: UV214
Mixer: ASI HyperShear In-Line 25 µL
HPLC Conditions:
MP: A = H2O, B = H2O doped with 0.05% red dye
Flow Rate: 50 µL/min.
Step Gradient: 0 to 100% B in 60 min., 10% steps
Linear Gradient: Same, 4 min. T0 hold
Figure 2
HPLC System Configuration:
ASI PrimeLine Model 500G Micro Head
Detection: UV214
Mixer: ASI HyperShear In-Line 150 µL
HPLC Conditions:
MP: A = H2O, B = H2O doped with 0.05% red dye
Flow Rate: 200 µL/min. Isocratic 35% B
Sample: Methyl and Ethyl Parabens

Figure 3
HPLC System Configuration:
ASI PrimeLine Model 500G Micro Head
Detection: UV214
Mixer: ASI HyperShear In-Line 25 µL
HPLC Conditions:
MP: A = H2O, B = H2O doped with 0.05% red dye
Flow Rate: 20 µL/min. Gradient 5 to 95% B in 20 min.
Sample: Methyl and Ethyl Parabens

